Recombinant Mouse Anti-DENV-3 Antibody (mAb 4E11)
CAT#: PABL-446
Recombinant Mouse Antibody (mAb 4E11) is capable of binding to DENV-3 , expressed in Chinese Hamster Ovary cells (CHO). This antibody is capable of neutralizing all four serotypes, and ourudy reveals the determinants of this cross-reactivity.
Published Data
Tested Data
Figure 1 4E11 blocks the binding of DE296–400 to model heparan sulfate groups.
4E11 blocks the binding of DE296–400 to model heparan sulfate groups. DE: the major dengue virus envelope glycoprotein.
Thullier, P., Demangel, C., Bedouelle, H., Mégret, F., Jouan, A., Deubel, V., ... & Lafaye, P. (2001). Mapping of a dengue virus neutralizing epitope critical for the infectivity of all serotypes: insight into the neutralization mechanism. Journal of General Virology, 82(8), 1885-1892.
❮
❯
ELISA
Figure 1 Mouse Anti-DENV-3 Recombinant Antibody (PABL-446) in ELISA
ELISA analysis of PABL-446 was performed by coating with DENV Envelop Protein, His tag. The HRP-conjugated anti-Mouse IgG was used as a secondary antibody.
WB
Figure 2 Mouse Anti-DENV-3 Recombinant Antibody (PABL-446) in WB
Western blot analysis was performed with 0.1 µg (lane 1), 0.3 µg(lane 2), and 0.6 µg (lane 3) antigen onto a 12% Tris-HCl polyacrylamide gel. Goat anti-Mouse IgG HRP secondary antibody at a dilution of 1:5,000 for one hour.
DB
Figure 3 Mouse Anti-DENV-3 Recombinant Antibody (PABL-446) in Dot Blot
Dot Blot analysis of PABL-446 was performed with DENV envelop protein.
PABL-446 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
SDS-PAGE
Figure 4 Recombinant Mouse Anti-DENV-3 Antibody (PABL-446) in SDS-PAGE
SDS-PAGE analysis of PABL-446 in non-reduced (Lane 1, 1.5 μg) and reduced condition (Lane 2, 1.5 μg).
SEC-HPLC
Figure 5 Recombinant Mouse Anti-DENV-3 Antibody (PABL-446) in HPLC
The purity of PABL-446 was greater than 90% as determined by SEC-HPLC.
❮
❯
Specifications
- Immunogen
- Dengue virus 3
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native DENV-3
- Species Reactivity
- DENV
- Clone
- mAb 4E11
- Applications
- WB 1:1000-1:10000
Neut Assay-depended
FuncS Assay-depended
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- Assay-depended
Target
- Alternative Names
- Dengue virus; DENV; Flaviviridae; dengue virus 3; DENV 3; Flaviviridae
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for PABL-446. Click the button above to contact us or submit your feedback about this product.
Minimal Background
One of this antibody's distinguishing qualities is its minimal background in experimental experiments. It generates clear and specific data, making analysis simple and dependable.
Good Packaging
The antibody arrived well-packaged, with all relevant information on the datasheet. The secure packaging guaranteed that the product was in perfect condition upon delivery.
Exceptional Performance
This antibody outperformed my expectations in terms of quality and performance. The constant results I get make it an extremely useful tool in my research arsenal.
Q&As
-
How can I dispose of unused or expired antibodies?
A: Follow your institution's guidelines for disposing of biohazardous materials. Typically, antibodies are disposed of in biohazard bags and then autoclaved or incinerated.
-
How do I calculate the molarity of my antibody solution?
A: Molarity (M) = (mass of antibody in grams) / (molecular weight of antibody in g/mol) / (volume of solution in liters). Use the antibody's molecular weight provided by the supplier.
-
What safety precautions are recommended when handling antibodies?
A: Wear appropriate PPE, including gloves and lab coats, handle in a biosafety cabinet if necessary, and follow proper disposal protocols for biohazardous materials.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABL-446, RRID: AB_3111658)
Copy citation
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "DENV-3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-443 | Recombinant Mouse Anti-DENV-3 Antibody Fab Fragment (PFBL-443) | WB, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-443 | Recombinant Mouse Anti-DENV-3 Antibody scFv Fragment (PSBL-443) | WB, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-486CQ | Recombinant Mouse Anti-DENV-3 Antibody (VC375) | LF | Mouse antibody |
| MRO-487CQ | Recombinant Mouse Anti-DENV-3 Antibody (MRO-487CQ) | ELISA, IF | Mouse antibody |
| MRO-488CQ | Recombinant Mouse Anti-DENV-3 Antibody (MRO-488CQ) | IF | Mouse antibody |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: FuncS, IF, Neut, ELISA, FC, IP, WB
Application: ELISA, IP, WB, IHC, IF, FuncS
-3.png)
Application: ELISA, FC, Block, SPR
![Figure 2 Anti-Human CD19 Recombinant Antibody Fab Fragment [TAB-1611CL-F(E)] in HPLC](https://img.creativebiolabs.net/productimages/COA-TAB-1611CL-F(E)-2.png)
Application: Depletion, FuncS
-CB2006C17L-4.jpg)
Application: WB, ELISA
Application: ELISA, IHC, FC, IP, IF, FuncS
-3.jpg)
Application: Neut
Application: ELISA, FC, Inhib
-2.png)
Application: WB, ELISA, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
